The system consists of:
Innovative Sample Preparation
• GDS uses proprietary magnetic particles to capture the target organism from the enriched sample.
• The innovative GDS PickPen® concentration device quickly and easily collects and transfers the concentrated target – 8 samples at a time.
• The sample preparation procedure is practical, efficient and simple to run.
Patented Probes and Primers
• Utilizes highly conserved target gene sequences.
• Ensures greater specificity with fewer indeterminate or false positive reactions.
Multiplex DNA Amplification
• Multiplex platform allows for the simultaneous detection of multiple targets within each amplification tube.
• Patented rotary format allows for faster cycling
The Assurance GDS system brings advances in speed, simplicity, specificity and sensitivity
SPEED
Faster than many conventional thermal cyclers, the Assurance GDS Rotor-Gene® is an innovative centrifugal air-exchange thermal cycler that puts a new spin on things.
• Its constant rotary motion overcomes the heat equilibrium issues inherent in conventional Peltier block systems and lowers dwell time, resulting in faster results.
• Save over 2 hours of amplification time per run with the Assurance® GDS cycler.
SIMPLICITY
From start to finish Assurance® GDS has been designed to be simple, flexible and easy to run.
• Requires no centrifuge or heating block.
• Eliminates associated steps and waiting periods.
• Not dependent on frequently ambiguous melt curve analysis after amplification to determine results.
SPECIFICITY
Assurance® GDS utilizes patented primers and probes to ensure greater accuracy.
Each amplification tube contains a set of highly specific primers/probes and a discrete internal control which ensure the highest level of specificity.
• Minimizes potential for cross-reactivity with non target organisms.
• Results in fewer indeterminate or false positive reactions.
• Provides greater confidence in results.
SENSITIVITY
Assurance® GDS delivers a greater amount of quality DNA for analysis, ensuring a higher degree of sensitivity.
• Its innovative patent-pending sample preparation procedure utilizes proprietary magnetic particles to capture and transfer the target organisms from the enriched sample to the amplification tube.
• Concentrated sample makes more DNA available for analysis allowing for a shorter enrichment.
Assurance® GDS Rotor-Gene:
- Rotor Capacity:
+ Black rotor – 36 wells for use with 0.2 mL amplification tubes
+ Blue rotor – 72 wells (sold separately) for use with 0.1 mL amplification tubes"
- Temperature range: Ambient to 99 °C
- Temperature accuracy: ±0.25 °C (type, measured 30 s after clock start)
- Temperature resolution: ±0.02 °C (smallest programmable increment)
- Temperature uniformity: ±0.01 °C
- Ramp rate: > 15 °C/s heating (peak ramp rates, air): > 20 °C/s cooling"
- Optical Specifications:
Excitation sources: High energy light-emitting diodes
Detector: Photomultiplier
Acquisition time: 4 s"
- Power Requirements:100 – 240 V AC, 50 to 60 Hz"
- Heat dissipation/thermal load:
+ Average: 0.183 kW (632 BTU/h)
+ Peak: 0.458 kW (1578 BTU/h)"
- The Assurance® GDS Rotor-Gene® Q software
+ display amplification curves for the “Test Samples” and “Internal Control” for each tube
+ Multiple assays can be run simultaneously on the Assurance® GDS Rotor-Gene®. Results for all assays appear in the results table by sample number.
+ Interpreting Results: Positive, Negative, No Amp"
+ User Levels: GDS Administrator, GDS Analyst, GDS Operator
