One 5 to 10 μL drop is placed on a specialized Videodrop slide within a magnetic sample holder. The operator prepares the stage for analysis by lifting the handle and lowering the sample protective shield. Preview mode confirms the Blue LED saturation is within parameters. The technician selects ‘record’ to initiate real-time analysis of the nanoparticle concentration and Brownian motion of the bioproduction batch. Cleaning occurs with a laboratory lint-free Kimwipe (Kimberly Clark) infused with certified detergents to absorb the drop on both the slide and objective lens. Data is readily available to review and export immediately after the desired number of particles are detected.
The Videodrop device aligns with the cell and gene therapy segment of the bioproduction market due to the correlation of collecting physical titer of viral vectors such as lentivirus, retrovirus, adenovirus, and extracellular vesicles (EVs) in the range of 80-500 nanometers. Videodrop outputs are linear compared to p24 ELISA, RT-qPCR, and NTA assays (Figure 1). The unit exhibits the ability to toggle from a nanoscale view to a microscopic view up to 10 μm to view the impurities of debris and aggregates. The Audit Trail software extension will allow users to track actions that have occurred within the software interface during operation accommodating GMP facility requirements. A Corning associate will set up and tune the equipment upon procurement. A 12-month Service Contract is optional and includes 1-annual Preventative Maintenance.
With its small footprint, ease of use, and processing speed, the Corning Videodrop is the ideal tool for characterizing nanoparticles and aims to accelerate clinical trials of drug, cell and gene therapy, and vaccine production.
Features
◗ Speed under 1 minute for
– Loading
– Processing
– Cleaning
◗ No calibration or wash is needed
◗ Repeatable and reproducible
– No inter-operator bias
– No parameter adjustment
